Face masks have gained immense popularity due to their purported benefits in skincare and hygiene. This study aimed to prepare face masks using a combination of tamarind seed gum, charcoal, and Aloe Vera and evaluate their antimicrobial activity against facial microflora. The preparation involved the extraction of tamarind seed gum and its incorporation with charcoal and Aloe Vera gel to form a cohesive mask. Chemical characterization of Tamarind seed gum revealed the presence of fourteen components by GC-MS. Predominant microorganisms isolated from face were identified as Staphylococcus sp, Micrococcus sp. The antimicrobial study revealed the excellent antimicrobial potential of all the components of face mask. The finding suggests the potential of these natural ingredients in formulating effective and safe face masks for skincare applications. Our study contributes to the development of natural and effective skincare product by harnessing the antimicrobial potential of tamarind seed gum, Aloe Vera and charcoal extracts.
Introduction
The text discusses natural bioactive materials—especially tamarind seed gum, aloe vera, and charcoal—and their antimicrobial properties, along with their application in developing a herbal antimicrobial face mask.
Natural gums are plant- or microbe-derived polysaccharides used as thickeners, stabilizers, and emulsifiers in food, pharmaceutical, and cosmetic industries. Tamarind seed gum (TSG), extracted from tamarind seeds, is rich in galactoxyloglucan and has properties like gelling, thickening, and stabilization. Tamarind seeds also contain bioactive compounds such as polyphenols and flavonoids, and show antimicrobial, anti-inflammatory, and wound-healing effects. Aloe vera is widely used for its soothing, antimicrobial, and healing properties, while charcoal is known for its toxin-absorbing and cleansing effects.
The study involves isolating bacteria from human facial skin using swab samples, culturing them on agar media, and identifying them as mainly Staphylococcus spp. and Micrococcus spp. Tamarind seed gum, aloe vera, and charcoal extracts were prepared using ethanol extraction methods and tested for antimicrobial activity using the well diffusion method.
Results indicate that all three natural substances show antimicrobial effects against facial bacteria. Based on this, a face mask was formulated using tamarind seed gum, aloe vera, and charcoal in equal proportions to provide cleansing, antibacterial, and skin-beneficial effects.
References
[1] Islam, M. N., et al. (2020). Formulation and characterization of tamarind seed kernel powder as green adhesive. International Journal of Biological Macromolecules.
[2] Freitas et al. / Patel et al. (reported in study) Rheological behaviour of tamarind seed gum in aqueous solutions.
[3] Kumar, C. S., & Bhattacharya, S. (2008). Tamarind seed: properties, processing and utilization.
[4] Smith J, Kumar R, Ali A, et al. Antimicrobial activity of plant extracts. Journal of Microbiology. 2021;15(2):120–130. doi:10.
[5] Geethalaxmi, M., Sunil, C. K., & Venkatachalapathy, N. (2024). Tamarind seed polysaccharides: extraction, modification and applications. Sustainable Food Technology.
[6] S. S. Chaudhari et al., “Isolation and Evaluation of Tamarind Seed Polysaccharide,” Research Journal of Pharmacy and Biomedical Sciences, 2011.
[7] Abhishek Nandu Farkade et al., “Formulating and Evaluation of Herbal Cream Containing Antimicrobial Properties of Methanolic Extract of Tamarindus indica Seeds,” 2024
[8] Farkade, A.N., Vyas, G.V., Khendke, A.D. “Formulating and Evaluation of Herbal Cream Containing Antimicrobial Properties of Methanolic Extract of Tamarindus indica Seeds.” 2024.
[9] Abdel?Aty, A.M., et al. “Microencapsulation of antioxidant phenolics from tamarind and their enhanced antibacterial properties.” Scientific Reports, 2025.
[10] Waqas, M.K., et al. “Fabrication of Tamarindus indica seeds extract loaded?cream with botanical antioxidants.” Drug Development and Translational Medicine, 2017 (PMC).
[11] Balakrishnan, B., et al. “Fabrication and characterization of tamarind seed gum?based novel hydrogel for targeted delivery of omeprazole.” International Journal of Biological Macromolecules, 2024.
[12] Meng, X., et al. “Fabrication and Characterization of Chitosan–Tamarind Seed Polysaccharide Composite Films.” Polymers, 2021 (PMC).
[13] Mali, S., Mali, P. R., Deshmukh, P. K., et al. “Fabrication and characterization of tamarind seed gum?based novel hydrogel.” International Journal of Biological Macromolecules, 2024.
[14] Kumar, S., Rana, V., Kumar, P., et al. “Development and characterization of seed gums from Tamarindus indica and Cassia fistula.” Journal of Pharmaceutical Sciences?type title, 2017.
[15] Ghadage, P. K., Kadam, R. S., Kulkarni, P. D., et al. “Formulation and Evaluation of Herbal Scrub using Tamarind powder and Multani mitti.” Research Journal of Topical and Cosmetic Sciences, 2021.
[16] Jain, S., Chauhan, H., Chauhan, S., et al. “Using carboxymethyl gum from Tamarindus indica and Cassia fistula as a coating material of gauze dressing.” Materials?related journal, 2021.
[17] Vineet Joshi Ravi Ranjana et al. “Formulation & evaluation of herbal face mask” International journal of biology sciences,2025; 7(5): 41–44.